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Journal: Metabolites
Article Title: GDF15 Improves Renal Injury Induced by Ectopic Lipid Deposition via AMPK/SIRT1 Pathway-Mediated Autophagy
doi: 10.3390/metabo16050336
Figure Lengend Snippet: GDF15 ameliorates renal ectopic lipid deposition and injury in obesity-resistant mice. ( A ) GDF15 protein expression in kidneys ( n = 3). ( B ) Serum GDF15 levels (n = 6). ( C – E ) Quantification of FAT/CD36, PLIN2, and KIM-1 protein expression in kidneys ( n = 3). ( F – H ) Oil Red O, H&E, and PAS staining of renal tissues (Scale bar = 50 μm). ( I ) Serum free fatty acid (FFA) ( n = 6). ( J ) Serum CPT-1 ( n = 6). ( K – M ) Serum levels of KIM-1, blood urea nitrogen (BUN), and creatinine (Cre) ( n = 6). ( N ) Homeostatic model assessment of insulin resistance (HOMA-IR) ( n = 6). Mean ± SD. * p < 0.05 and ** p < 0.01 vs. the NC group; # p < 0.05, ## p < 0.01, and ### p < 0.001 vs. the DIO group.
Article Snippet: The levels of free fatty acids (Abbkine, Wuhan, China), creatinine (Cre) (Nanjing Jiancheng, Nanjing, China),
Techniques: Expressing, Staining
Journal: Metabolites
Article Title: GDF15 Improves Renal Injury Induced by Ectopic Lipid Deposition via AMPK/SIRT1 Pathway-Mediated Autophagy
doi: 10.3390/metabo16050336
Figure Lengend Snippet: Blockade of the GDF15-GFRAL pathway induces glucose and lipid metabolic disorders and renal dysfunction in mice. ( A ) Schematic diagram of the animal grouping and treatment protocol. ( B ) GFRAL mRNA expression levels ( n = 3). ( C ) Mouse body size, kidney appearance, kidney weight ( n = 6), and percentage of kidney weight to body weight ( n = 6). ( D , E ) Serum free fatty acids (FFA) and CPT-1 levels ( n = 6). ( F , G ) Serum glucose and insulin levels ( n = 6). ( H ) HOMA-IR ( n = 6). ( I – K ) Serum levels of KIM-1, blood urea nitrogen (BUN), and creatinine (Cre) ( n = 6). Mean ± SD. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. the shControl group.
Article Snippet: The levels of free fatty acids (Abbkine, Wuhan, China), creatinine (Cre) (Nanjing Jiancheng, Nanjing, China),
Techniques: Expressing